Members of the county council’s planning committee voted by six to five to refuse planning permission to UK Oil & Gas plc (UKOG) for its proposed Loxley wellsite.
They said the proposal contravened local planning policy on transport and landscape grounds.
There was also concern that the wellsite would damage nearby local businesses, particularly a wedding venue, craft brewery and farm that hosts a cancer-awareness festival.
A previous planning committee on 29 June 2020 voted to refuse by the same margin. That decision was later rescinded after technical problems with the online meeting.
On both occasions, councillors went against the advice of planning officers who had recommended approval of the application.